Basic Laboratory Techniques Safety And Hematocrit

Basic research laboratoryoratory Techniques Safety And haematocritThe hematocrit is unremarkably orde cherry as a part of the complete demarcation librate. It is cardinal in evaluating anemia and polycythemia, monitoring the recovery from dehydration, the effectiveness of treatment for anemia, the on-going bleeding to check its severity, etc. In this practical, hematocrit is utilize to gibe if the patient has anemia by closes of red stall indices MCV, MCH and MCHC. For red air cell count, it is used to evaluate any type of decline or increase in the number of red dividing line cells as measured per liter of line of products. It is a parameter interprets in conjunction with hematocrit. some(pre noinal) decreased hematocrit and decreased red blood cell count indicate anemia.(D) Resultspacked cell volume (HCT)Record the red blood cell indices in the table below. versionSI Uniterythrocyte8.55 - 1012RBC/LHemoglobin11.0g/dLYour HCT value26%The red blood cell indices be us ed to classify anemias. Find out their formulae and calculate the MCV, MCH MCHC. Compare them to the cite value of a normal adult female.Red cell indices locutionCalculation (units)Reference range involve cell volume (MCV) hematocrit (%) - 10RBC_26 - 108.55MCV= 30.4 fL86 98 fLMean cell hemoglobin (MCH)Hemoglobin(grams) - 10RBC__11__ - 108.55MCH= 12.87 pg27 32 pgMean cell hemoglobin concentration(MCHC)Hemoglobin(grams) - 100Hematocrit (%)__11__ - 10026MCHC= 42.3 %32 37 %(*delete as appropriate and suggest whether blood smear A or B provided in the lab would perish to this sample)As your reason MCV suggest, the RBCs are macrocytic / normocytic / microcytic *.As your calculated MCH suggest, the hemoglobin in the RBCs are within / below / above* the average weight.As your calculated MCHC suggest, the RBCs are normochromic / hypochromic / hyperchromic*. ancestry smear A / B* belongs to this patient.Cell count and hemacytometerRBC CountsAveraging no. of RBCs in 2one square millime terSquareNo. of RBC counted11782164Total RBCs counted342Averaged RBC counted171Calculate the RBC count in the sample= Average no. RBCs counted in 1 big square - Dilution factorArea counted (mm2) - deepness (mm)RBC count = 171 - 1000 ikikikiiiiiiu0.2 (mm2) - 0.1 (mm)RBC count = 8.55 - 1012 (units= /L )(E) tone of voice AssessmentObtain results from the class and do the statistics.(Refer to Basic Clinical Lab Techniques pp. 79 81)X_n(117+160+174+142+166+161+181+183+166+143+164+159+148+162+225+135+clxv+120+168+128+271+104+171+168+171+182+one hundred seventy+176+181+116+172) 31RBC counts is 162.87 .nTest Value(mg/dL)X variance Squared( x)211172104.1521608.243174123.864142435.6051669.7961613.507181328.668183405.1891669.7910143394.86111641.271215914.9813148221.15141620.76152253860.0116135776.79171654.53181201837.921916826.31201281215.982127111691.89221043465.792317166.082416826.312517166.0826182365.922717050.8228176172.3729181328.66301162196.893117283.34( x)2__n 130297.48__30= 1009 .916Variance1009.91631.782s = 63.56+2s = 226.43-2s = 99.313s = 95.34+3s = 258.21-3s = 67.5310. social organization of a Levey-Jenning s Chart using the mean and standard deviation(s) from above. presage the mean value, +/- 1s, +/-2s, +/-3s on the appropriate lines. Using the RBC data from the class, plan the values from all students.Levey-Jennings Chart of RBC counts in classSD of RBC counts(G) Practical Review QuestionsLabel the hemacytometer diagram below with its parts. (p208) Indicate the areas usually used forWBC count with WRBC count with R andPlatelet count.p1. What does the hematocrit measure?The hematocrit is a test find the patients red cell volume found in whole blood and, thus, the bloods oxygen carrying capacity. It is expressed as a office by volume.2. score the hematocrit reference values for males, females, and newborns.Males 42 52 %Females 36 48 %Newborns 51 61 %3. Name a condition that could cause a decreased hematocrit value.Anemias4. Explain the hemato crit procedure1. Obtain patients blood and prepare the specimen.Gently mix the capillary blood by inverting the thermionic valve until all sediments disappear.Blood sample is drawn into a heparinixed microhematocrit provide by capillary follow up. Load the microhematocrit furnish by holding it end downwardly to allow gravity to facilitate loading of the subway system. The pipe should be alter to about 3/4 full.Seal one end of the provide with a bittie amount of clay material at a 90 angle.2. Centrifuge the samples.Please the close microhematocrit tube into the rotor of the microhematocrit centrifuge, with the sealed end against the rubber gasket.Duplicate samples should be placed opposite apiece other in order to balance the centrifuge.Securely fasten the mo nonone lid on covert of the capillary tubes.Spin the tubes for 2 to 4 minutes at 10000 rpm.After the centrifuge has s peakped, open the top and remove the cover plate.3. Reading and reporting the Hematocrit value.I nspect the tube to see if there is any leakage after centrifugation.Place the tube on the hematocrit reader.Place tube in the groove with the top of the seal-ease (bottom of the packed cells) lined up with the bottom line of the reader.Move the slider bar to the border between the packed red cells and the plasma.Read the percentage reading beside the slide bar.5. Blood enters the capillary tube by what action?Blood enters by capillary action.6. Why must the capillary tube be sealed securely?In the process of spinning, RBCs and a small amount of plasma will be forced from the tube, a fictitious result may be resulted because of incomplete sealing of tube. Therefore, sealing the tube securely can create a pressure inside the tube which prevents the content from forcing out during centrifugation.7. What is the usual length of time for centrifugation of the hematocrit tubes?The hematocrit tubes are usually centrifugated for 2 to 4 minutes at 10000rpm.8. What arctic perplexitys shoul d be observed when performing a hematocrit?Standard precaution should be observed.9. What technical factors can affect the quality of hematocrit results? Blood sample should be well-mixed before microhematocrit tubes are filled. Avoid bubbles when filling blood sample into the microhematocrit tube. Microhematocrit tubes should be filled at least three-fourths full. The microhematocrit tubes should be completely sealed before centrifugation. Read the hematocrit value at the top of the red cell layer, not at the top of buffy coat.10.Case body of work 1A hemoglobin and hematocrit test performed in a pediatric clinic on a 2-year-old boy gave results of 110 g/L hemoglobin and 0.33 L/L hematocrit. Do these results agree? What is the boys general health status based on the hematology results?From the data provided by the case, the mean cell hemoglobin concentration (MCHC) of the child__11__ - 10033= 33%The MCHC is within the normal range, showing the child is free from anemia.However, ac cording to homo Health Organization, a hemoglobin concentration of less than 110 g/L and a hematocrit value of less than 33% were used as a cut-off for anemia for children aged 0.5 to 51.Therefore, for the above case, although the child does not have anemia at this moment, his marginal hemoglobin and hematocrit values agree and both show that he has a high endangerment of suffering anemia.Age or gender groupHemoglobin (g/dL)Hematocrit (%)Children (0.5-4)Children (5-12)Children (12-15)Adult MenNon-pregnant WomenPregnant Women